Keeping this in consideration, what enzymes are in Accutase?
Accutase is a natural enzyme mixture with proteolytic and collagenolytic enzyme activity. This means it mimics the action of trypsin and collagenase at the same time.
Furthermore, what does Accutase cleave? Accutase gently and rapidly dissociates tissues for cell isolation and propagation. It is recommended for use with SmartPlastic adhesion cell culture plasticware Accutase combines protease and collagenolytic activities which maximizes its versatility for cell detachment and tissue dissociation.
Considering this, does Accutase contain EDTA?
1 x Accutase enzymes in Dulbecco's PBS (8.0 g/L NaCI, 0.2 g/L KCl, 0.2 g/L KH2PO4•4Na and 1.15 g/L Na2HPO4) and 0.5 mM EDTA•2Na.
How do you inactivate Accutase?
Leaving a bottle in a 37°C water bath until it is visibly defrosted could degrade the performance of Accutase® as it will be inactivated after 1 hour at 37°C. It is therefore recommend that Accutase® be defrosted overnight in the refrigerator or in a bath of cold water.
